Measure
We can monitor molecules in complex samples or in single cells, from chemicals to metabolites, RNA or proteins. We have sensitive assays to measure the impact of molecules on biological functions, from growth to toxicity to endocrine disruption.
Methods
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Effect Directed Analysis (EDA)
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Analytical chemistry
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(Cell) Biology
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Single-cell
Effect Directed Analysis (EDA)
In effect-directed analysis, we combine chromatography for fractionation of complex samples, and split these fractions for simultaneous functional assessment (cell-based assay) and compound identification (using mass spec).
In this way we can identify bioactive compounds in complex samples such as environmental samples, snake venoms, foods or body fluids.
Analytical chemistry
Even the all-powerful Pointing has no control about the blind texts it is an almost unorthographic life One day however a small line of blind text by the name of Lorem Ipsum decided to leave for the far World of Grammar. The Big Oxmox advised her not to do so, because there were thousands of bad Commas, wild Question Marks and devious Semikoli.
Techniques
Mass spec-based methods
We have advanced analytical methods for metabolomics, proteomics and glycomics for the analysis of human fluids, intracellular metabolites, tissues and cell culture media. We hyphen separation techniques as HPLC, CE and GC with high-end (tandem) mass spectrometry (high-resolution, ion mobility and UVPD/IRMPD). We also have MS-based imaging facilities.
Contacts: Pim Leonards, Govert Somsen, Sofia Moco
NMR and crystallography
We have 300 – 600 MHz NMR machines for compound identification and analysis of complex fluids for metabolite quantification.
For protein structure determination, the workflow includes liquid handling for sample crystallization, optimizing crystals, crystal handling including cryo-protection and storage. Diffraction analysis and data collection is done on an in-house, state of the art Bruker D8 Quest X-ray source attached to an cutting edge PhotonII detector including downstream software suits for structure determination.
Contact: Elwin Janssen (nmr.beta@vu.nl).
Techniques
Congratulations to Elisabeth Naderlinger, Lenya de Brouwer, and Olivia Klatt for winning the MIMETAS-AIMMS VU OrganoPlate® Platform Proposal Contest! 🎉 The idea for the MIMETAS-AIMMS…
When ten bright minds come together for two full days, innovation is bound to prosper! The first AIMMS hackathon revolved all around finding alternative protein…
(Cell) Biology
Even the all-powerful Pointing has no control about the blind texts it is an almost unorthographic life One day however a small line of blind text by the name of Lorem Ipsum decided to leave for the far World of Grammar. The Big Oxmox advised her not to do so, because there were thousands of bad Commas, wild Question Marks and devious Semikoli.
Single-cell
Even the all-powerful Pointing has no control about the blind texts it is an almost unorthographic life One day however a small line of blind text by the name of Lorem Ipsum decided to leave for the far World of Grammar. The Big Oxmox advised her not to do so, because there were thousands of bad Commas, wild Question Marks and devious Semikoli.
Sample projects
A team led by microbiologist Edith Houben at Vrije Universiteit Amsterdam has secured an ERC Synergy Grant, worth over €10 million, to study tuberculosis bacterium's…
Chemist Ivana Drienovská receives the Vidi for her research Greening Our World: Unlocking Enzymatic Solutions for the Fluorination Reaction. For decades, scientists have marveled at the remarkable catalytic…
The AIMMS MSc Talent program – is a unique training program for talented Master students in the field of molecular life sciences. What’s in it…
Are you ready to dive into the world of computational chemistry? This five-day course equips you with the theoretical knowledge and practical skills necessary to…
Trevor A. Hamlin Assistant Professor Faculty of Science, Chemistry and Pharmaceutical Sciences "My three-year-old daughter is my world...we love doing active things together." Can you…
Dr. Mr. Paul Nederkoorn has been appointed by the Vrije Universiteit (VU) as an endowed professor for the chair ‘Valorization of Science and Technology’ within…